Maggots did not appear in the covered jars because the jars were sealed, preventing flies from accessing the contents to lay eggs. This experiment, often associated with Francesco Redi's work in the 17th century, demonstrated that spontaneous generation was not responsible for the formation of maggots; instead, it showed that they originated from eggs laid by adult flies in open environments. The closed jars created a barrier, eliminating the possibility of maggot development.
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