Can the nesting box be removed from the cage after the babies are moving and alert?

DO NOT remove the nesting box at all for at least 4 weeks after the litter of babies has been born. It is very important to keep the nesting box as untouched as possible until at least 4 weeks! Though the babies are out and about they will still spend much time cuddling inside (though they won't always) You may not see them in it much during the day but it is very important for them at night and is very important to stay untouched for it is where the mother will go to feed them until they are weened at 6-8 weeks