Yes you can and you often have to if you are using a crate to house train a young puppy. Some crates come with a divider panel that you can place where ever you need it in the crate depending on the size of your dog. If yours does not have a panel you could use a piece of plywood cut to fit inside. Sometimes if the space is much bigger than the dog they will still eliminate in the crate. Dividing it and making the space smaller usually corrects this.
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