Why is the Elisa test so sensitive and specific?

The ELISA (Enzyme-Linked Immunosorbent Assay) test is sensitive and specific due to its ability to utilize antibodies that bind to specific antigens, allowing for the detection of even low concentrations of target molecules. The test employs a solid surface to immobilize the antigen or antibody, enhancing the stability and accessibility of the target. Additionally, the use of enzyme-linked detection amplifies the signal, increasing sensitivity. Overall, the combination of high affinity antibodies and signal amplification contributes to its reliability and accuracy in diagnosing various conditions.