How to use the Roto Brush tool effectively in Adobe After Effects?

To use the Roto Brush tool effectively in Adobe After Effects, first select the tool from the toolbar and then click and drag to create a rough selection around the object you want to isolate. Refine the selection by adjusting the brush size and using the refine edge tool. Make sure to preview your selection frequently to ensure accuracy. Once you are satisfied with the selection, you can apply effects or make adjustments to the isolated object.