What makes the biurette test?

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2026-03-15 11:40

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The biuret test is a chemical assay used to detect the presence of proteins in a solution. It involves adding a biuret reagent, which contains copper sulfate, to the sample. If proteins are present, the solution changes color, typically to a violet or purple hue, due to the formation of a complex between the copper ions and the peptide bonds in the proteins. The intensity of the color change can also indicate the concentration of protein in the sample.

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