The standard tuning for an acoustic guitar is EADGBE. To properly tune your guitar, start by tuning the low E string to a reference pitch, then use the fifth fret method to tune the A, D, and G strings. For the B string, use the fourth fret method, and for the high E string, use the fifth fret method again. Make sure each string is in tune by comparing it to the reference pitch or using a tuner.
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