Test for bilirubin level in amniotic fluid can be invalidated by?

The bilirubin level test in amniotic fluid can be invalidated by factors such as contamination with maternal blood, which can artificially elevate bilirubin levels. Additionally, the timing of the sample collection relative to the rupture of membranes can affect the results, as bilirubin may diffuse from the fetal blood into the amniotic fluid over time. Proper handling and processing of the sample are crucial to ensure accurate results.