The Benedict's test has several disadvantages, including its inability to specifically differentiate between different types of reducing sugars, as it reacts with all reducing sugars, not just glucose. Additionally, the test is semi-quantitative, providing only an estimate of sugar concentration rather than an exact measurement. It is also sensitive to factors like pH and temperature, which can affect the results. Lastly, the test can yield false positives due to the presence of other substances, such as certain amino acids and vitamins, that can reduce copper ions.
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