How do you test fluorescence?

Fluorescence testing typically involves exposing a sample to a specific wavelength of light, which excites the electrons in fluorescent molecules, causing them to emit light at a longer wavelength. This emitted light is then detected using a fluorescence microscope or a spectrofluorometer. The intensity and wavelength of the emitted fluorescence provide information about the concentration and characteristics of the fluorescent compounds in the sample. Proper controls and calibration are essential to ensure accurate and reliable results.