Classical genotyping refers to traditional methods used to determine the genetic constitution of an organism, typically through techniques like PCR (polymerase chain reaction), gel electrophoresis, or restriction fragment length polymorphism (RFLP). These methods analyze specific DNA sequences to identify genetic variations such as single nucleotide polymorphisms (SNPs) or other markers. Classical genotyping is often used in research, clinical diagnostics, and breeding programs to assess genetic diversity, disease susceptibility, or trait inheritance. While more advanced techniques like next-generation sequencing (NGS) have emerged, classical methods remain foundational in genetic studies.
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