How many times can a puffer fish puff up before dying?

A pufferfish can puff up multiple times as a defense mechanism, but the exact number varies among species and individual fish. Each time it inflates, it stresses its body and depletes energy reserves. While there is no specific limit to how many times a pufferfish can puff up, excessive inflation can lead to health issues and may ultimately shorten its lifespan. Thus, while they can puff up several times, repeated inflation can be detrimental over time.