What is the most accurate way to determine the gender of a kitten that is 2 weeks old?

The most accurate way to determine the gender of a 2-week-old kitten is by looking at the genital area. Male kittens will have a small distance between the anus and the genital opening, while female kittens will have a larger distance and may also have a small slit. It is best to have a veterinarian confirm the gender for certainty.