A greater tension during tetanus compared to a single twitch is primarily due to the increased frequency of stimulation. In tetanus, rapid successive stimuli prevent the muscle from fully relaxing between contractions, leading to a cumulative effect of calcium ions in the muscle fibers that enhances contraction strength. This sustained release of calcium allows for a more prolonged interaction between actin and myosin filaments, resulting in stronger and more sustained muscle contractions. Thus, the overlapping contractions lead to a greater overall force than a single twitch can generate.
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