Why do you isolate from sections obtained at the advancing area of the lesion?

Isolating sections from the advancing area of a lesion is crucial because this region often contains the most active and aggressive tumor cells, which can provide insights into the biological behavior of the tumor. Analyzing these sections can help identify specific molecular and genetic characteristics, aiding in prognosis and treatment decisions. Additionally, it allows for a better understanding of the tumor microenvironment and its interactions with surrounding tissues. Overall, focusing on the advancing edge enhances the accuracy of diagnosis and therapeutic targeting.