It depends on the dictionary, and the criteria you apply to what kinds of Words are considered to qualify. The spelling out of chemical names, for example, can result in Words of truly eye-watering length (titin, a protein, runs to 189,819 letters!), but are rarely counted among "long Words" in the English language.
This is a popular question, however, and consensus has established some definite front-runners in the Longest Word in the English Language (by letter count, which is a dispute in itself) category.
In one of his comedies, Aristophanes coined a Word as the name of a fictitious foodstuff that was 171 letters long in Greek, and 183 letters long when transliterated into English. The longest Word to appear in Shakespeare's works (honorificabilitudinitatibus) is 27 letters long.
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