Will the tune port setup work on a 400 small block?

Yes, a tune port setup can work on a 400 small block, provided that the engine has been modified to accommodate the specific requirements of the intake system. You may need to ensure compatibility with the throttle body, fuel injectors, and other components. Additionally, proper tuning will be essential to optimize performance. However, it's always best to consult with a professional or do thorough research specific to your setup for best results.