How do you collect fresh fractional urine specimen?

There should be a port at the end of the catheter where the tubing attaches to the catheter bag. Wipe with alcohol and, with a needle and syringe, insert the needle into the port and withdraw 10cc-20cc or so. Without a needle, you can take it from the bag itself, but the sample won't be as pure. You can also disconnect the catheter from the tubing and extract a sample, but this increases infection risk. If so, get a sterile container, wipe the entire area where the catheter meets the tubing with alcohol, put the catheter into the container, and hopefully you'll get about 5cc-10cc. When you reconnect, be very careful not to touch the inside of the catheter. This line runs straight into the person's bladder, and thus is a risk for bladder infection.