If IRS charged me for early withdrawal of my 401K will I be taxed again when I file my federal income tax?

Yes. That was the penalty for early withdrawal. What you received is then taxed as income received (which it would be whenever you received it). You did not pay tax on it when you put it in the account. Now more than likely, you actually only had something withheld on withdrawal, like withholding on pay. The penalty and tax on income will be calculated with your return, where you'll then take a credit for the amount withheld and true up to what the total actual tax and penalty is. I would point out that in all distributions of this type it was explained to you several times and you likely signed several documents explaining what would happen and you understood it. The Co paying it out was required to withhold I believe at 20%, the penalty is not that much, but the income tax, especially if it a substantial withdrawal putting you in a higher than normal tax bracket that year, will frequently be higher than the 10% left.