What divided the northwest territory into two parts?

The Northwest Territory was divided into two parts primarily by the passage of the Ohio Enabling Act in 1802, which allowed for the creation of the state of Ohio. This act established a boundary that separated the area that would eventually become Ohio from the remaining territory. Additionally, the 1787 Northwest Ordinance laid the groundwork for the governance and eventual statehood of territories in the region, influencing how they were divided and organized.