What is differencess between start up cost and operating cost?

Start up costs are the things you are going to need to get the business giong i.e. premises, furniture, IT, registration with local authorities, stock / materials etc. These are mainly one off payments that do not reoccur.

Operation costs are the day to day costs of running the business usually calculated in terms of monthly or annual costs. These costs will be for things like power, staffing, materials etc.