What is shortest interstate route from Macon to Jacksonville?

The shortest interstate route from Macon, Georgia, to Jacksonville, Florida, is primarily via I-75 South and I-295 East. Begin on I-75 South, then take I-295 East around Jacksonville, which connects to I-95 South leading into the city. This route typically covers around 170 miles and takes approximately 2.5 to 3 hours, depending on traffic conditions.