During mitosis, sister chromatids, which are identical copies of a chromosome, separate during anaphase. The mitotic spindle, composed of microtubules, attaches to the centromeres of the chromatids and pulls them toward opposite poles of the cell. This separation ensures that each daughter cell receives an identical set of chromosomes, maintaining genetic consistency. Proper functioning of the spindle is crucial for accurate cell division and the prevention of genetic disorders.
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