What was the first conflict in the Americas that the colonists fought without the aid of colonial powers?

The first conflict in the Americas that the colonists fought without the aid of colonial powers was the Pequot War, which took place between 1636 and 1638. This conflict involved English settlers in New England and the Pequot tribe, resulting from competition over trade and territorial disputes. The war ended with a significant defeat for the Pequot, leading to their near destruction and the establishment of English dominance in the region.