What structual differences suggest that eukaryotic cells evolved from prokaryotic cells?

Eukaryotic cells possess membrane-bound organelles, such as the nucleus, which separates genetic material from the cytoplasm, a feature absent in prokaryotic cells. Additionally, eukaryotic cells have a more complex cytoskeleton and larger ribosomes compared to prokaryotes. The endosymbiotic theory further supports their evolution, suggesting that certain organelles, like mitochondria and chloroplasts, originated from free-living prokaryotes that were engulfed by ancestral eukaryotic cells. These structural differences indicate a significant increase in complexity, supporting the evolutionary transition from prokaryotic to eukaryotic life forms.