How to make mannitol standard of 1ppm 10ppm 100ppm 500ppm and 1000ppm diluted in deionized water to generate a linear standard curve and need to make a total assay of 0.2 ml each from the stock?
take 1mg (0.001g) of mannitol and dilute it by deionised water to 100ml in 100ml measuring flask.The resulting solution is 10ppm.and then make further dilution to prepare required concentration.
