What countries did the pope's line of demarcation greatly affect why was it important?

The Pope's line of demarcation, established by the Treaty of Tordesillas in 1494, primarily affected Spain and Portugal by dividing newly discovered lands outside Europe between the two nations. This division was significant as it shaped colonial expansion and territorial claims in the Americas and other regions, influencing the historical development of these countries and their empires. It also set a precedent for European powers in navigating issues of exploration and colonization, ultimately impacting global trade and cultural exchanges.