The key steps involved in conducting a Mosher ester analysis include:
- Preparing the sample by synthesizing the Mosher esters from the compound of interest.
- Analyzing the sample using NMR spectroscopy to determine the chemical shifts of the protons in the Mosher esters.
- Comparing the chemical shifts of the protons in the Mosher esters with a reference database to determine the absolute configuration of the original compound.
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