How far apart do the red and green signals have to be when scoring a break-apart probe as POSITIVE?

When scoring a break-apart probe as positive, the red and green signals typically need to be separated by a minimum distance of 1.5 to 2 micrometers. This distance indicates that the two signals originate from distinct genomic locations, suggesting a successful detection of the target sequence. The exact distance may vary depending on the specific assay and experimental conditions used.