What is the use of sandwich elisa?

Sandwich ELISA (Enzyme-Linked Immunosorbent Assay) is a highly sensitive technique used to detect and quantify specific proteins, such as antigens or antibodies, in complex samples. It involves capturing the target molecule between two antibodies: a capture antibody coated on the plate and a detection antibody that is linked to an enzyme. The presence of the target protein is determined by adding a substrate that produces a measurable signal, typically a color change, proportional to the amount of the target present. This method is widely used in clinical diagnostics, research, and quality control in various fields.