What is the principle involved in the isolation of globulin?

The isolation of globulin involves the principle of solubility differences among proteins. Globulins can be precipitated from a solution by adjusting the ionic strength, pH, or temperature, which alters their solubility. Common methods include ammonium sulfate precipitation or using specific solvents to selectively precipitate globulins while leaving other proteins in solution. This selective precipitation allows for the concentration and purification of globulin from complex mixtures.