The sequence of bases in RNA is determined by the DNA template during the process of transcription. RNA polymerase binds to a specific region of the DNA and synthesizes a complementary strand of RNA by matching RNA nucleotides (adenine, uracil, cytosine, and guanine) to their corresponding DNA bases (thymine instead of uracil). This sequence ultimately encodes the information needed for protein synthesis. Thus, the RNA sequence is a direct reflection of the gene it transcribes from the DNA.
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