How do you prepare PBS?

To prepare phosphate-buffered saline (PBS), dissolve 8 g of sodium chloride (NaCl), 0.2 g of potassium chloride (KCl), 1.44 g of disodium hydrogen phosphate (Na2HPO4), and 0.24 g of sodium dihydrogen phosphate (NaH2PO4) in approximately 800 mL of distilled water. Adjust the pH to 7.4 using hydrochloric acid or sodium hydroxide as needed. Once the pH is correct, bring the final volume to 1 liter with distilled water, and sterilize by autoclaving or filter sterilization if required.