Deionised water for setting to zero of absorbance?

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1221966

2026-05-15 02:30

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Deionised water is commonly used as a blank solution for setting the absorbance to zero in spectrophotometry. This is essential because it allows for the calibration of the instrument by eliminating any absorbance caused by impurities or the solvent itself. By using deionised water, which has had its ions removed, the baseline measurements become more accurate, ensuring that any absorbance readings from samples reflect only the analyte of interest. This practice is crucial for obtaining reliable and reproducible results in quantitative analysis.

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