How does behaviour of indicate EBT changes with pH?

The behavior of Electrophoretic Mobility (EBT) changes with pH due to the alteration of the surface charge of particles in a solution. As pH increases, the ionization of functional groups on the particle surface can lead to a more negative charge, enhancing negative mobility. Conversely, at lower pH levels, these groups may become protonated, resulting in a reduced or even positive charge, which can decrease negative mobility. Thus, EBT is highly sensitive to pH variations, affecting particle interactions and movement in an electric field.