What is one of the following violations will result in a two-stroke penalty?

One violation that results in a two-stroke penalty in Golf is when a player fails to properly take relief from an obstruction or hazard. For instance, if a player incorrectly drops the ball in a designated relief area, they will incur a two-stroke penalty for not following the proper procedure. Additionally, if the player does not play the ball as it lies when required, they can also receive a two-stroke penalty.