What is the method for pasteurized milk serial dilution?

It is very simple. You just boil the water.

Sorry..... you boil the milk. It kills the germs.

Well that is an awful answer.

What you do is:

1. Using a sample of milk, transfer 1ml into the first sterile water blank. Label this tube 1:10.

2. Mix the tube

3.Using another transfer pipette, transfer 1ml of the 1:10 tube to the next sterile blank tube. Label this tube 1:100.

4.Continue making transfers until 1:100 and 1:10000 dilutions have been made.

5. After all dilution have been completed, transfer ½ ml from the 1:10 dilution and place it on a plate labeled 1:20.

6. Spread the liquid across the surface of the plate with a clean spreading rod.

7. Continue making plates in this fashion from each of the dilution tubes until you have created four plates: 1:20, 1:200, 1:2000, 1:20000.

8. Place the plates to be incubated.