What keeps the strands of DNA separate during replication?

During DNA replication, single-strand binding proteins (SSBs) play a crucial role in keeping the strands of DNA separate. Once the double helix unwinds, these proteins bind to the exposed single-stranded DNA to prevent it from re-annealing or forming secondary structures. This stabilization allows the DNA polymerase to synthesize new strands accurately and efficiently. Additionally, the unwinding of the DNA is facilitated by helicase enzymes, which separate the two strands.