What is the value of a 1953 US 2 dollar bill with a red seal?

It depends on its condition and the series letter, if any, after the date.

there are 4 different letter possibilities. As of 05/2009, auction values for bills in circulated condition are approximately:

No letter by the date: $2.25 - $2.50

"A" next to the date: $2.25 - $5.00

"B": $2.75 - $5.50

"C": $3.50 - $6.50

Please see the Related Question

Serial numbers are just counters and rarely help to identify a bill. The most important factors are normally the date, seal color, and letter (if any) next to the date.