Which colony did the English make their first attempts at an American colonization on?

The English made their first attempts at American colonization in the Roanoke Colony, established in 1585 on Roanoke Island in present-day North Carolina. Sponsored by Sir Walter Raleigh, this initial venture faced numerous challenges, including food shortages and hostile relations with Indigenous peoples. The colony ultimately disappeared, leading to the mystery of the "Lost Colony." Subsequent successful colonization efforts came with the establishment of Jamestown in 1607.