How chloroform makes three phases of RNA DNA and protein in phenol chloroform extraction?

Chloroform is a solvent that helps to separate the three phases in phenol-chloroform extraction by disrupting the interactions between the biomolecules. RNA, DNA, and proteins have different affinities for phenol, chloroform, and water, leading to their partitioning into separate phases based on their solubility. Chloroform enhances the separation by forming distinct layers that can be easily separated, allowing for the isolation of the desired biomolecules.