How did filippo pacini discovered cholera?

Filippo Pacini, an Italian pathologist, discovered the cholera bacterium, Vibrio cholerae, in 1854 while investigating an outbreak of cholera in Florence, Italy. He observed the bacteria in stool samples from infected patients using a microscope, identifying its characteristic shape and motility. Pacini's work laid the groundwork for understanding the disease's microbiological basis, although it wasn't widely recognized until later, when Robert Koch confirmed the discovery in 1883.