What would occur if an orange shield tube is collected before a light blue stopper?

If an orange shield tube is collected before a light blue stopper, it may lead to improper sample handling or contamination. Depending on the context, this could affect the validity of the results, as the order of collection often ensures that additives in the tubes interact correctly with the sample. Additionally, it might compromise the integrity of the specimen, potentially skewing diagnostic outcomes. Following proper protocol for sample collection is crucial for accurate testing.