Which western film was the first to use the song 'home on the range'?

The first western film to use the song "Home on the Range" was "The Old Chisholm Trail," released in 1941. The song, originally written in the 1870s, became closely associated with the western genre and has since been featured in numerous films and adaptations. Its inclusion in this film helped solidify its status as a classic cowboy Ballad.