In the determination of crude fiber, the hydrolyzed sample must be in contact with a hydrolyzing agent to break down complex carbohydrates such as cellulose and hemicellulose into simpler components. This process allows for the separation of insoluble fiber from soluble fiber, enabling accurate measurement of crude fiber content. Without proper hydrolysis, the fiber components may not be effectively separated and quantified, leading to inaccurate results.
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