What was Osceola's first act of defiance against the federal government relocation plan?

Osceola's first act of defiance against the federal government’s relocation plan occurred in 1835 when he openly resisted the Treaty of Fort Gibson, which aimed to remove the Seminole people from their ancestral lands in Florida. He organized and led a group of Seminoles in a rebellion against U.S. forces, refusing to abandon their territory. This act of resistance marked the beginning of the Second Seminole War, as Osceola became a prominent leader in the fight against forced removal.