How many total laborers arrived in Jamestown in the three groups?

A total of approximately 500 laborers arrived in Jamestown in three primary groups between 1607 and 1609. The first group included about 100 settlers in 1607, followed by another 100 in 1608, and finally, around 300 more in 1609 as part of the third supply mission. These laborers were sent to establish and sustain the colony, which faced numerous challenges in its early years.