What is the method used to separate bacteria into two groups based on cell wall composition called?

The method used to separate bacteria into two groups based on cell wall composition is called the Gram staining technique. This method categorizes bacteria into Gram-positive, which have a thick peptidoglycan layer in their cell walls and retain the crystal violet stain, and Gram-negative, which have a thinner peptidoglycan layer and do not retain the stain, appearing pink after counterstaining. Gram staining is a fundamental procedure in microbiology for identifying and classifying bacterial species.