What step of cell cycle does the separation of the cytoplasm occur?

The separation of the cytoplasm occurs during the final stage of the cell cycle known as cytokinesis. This process follows mitosis, where the cell's nucleus divides, ensuring that each daughter cell receives a complete set of chromosomes. Cytokinesis involves the formation of a cleavage furrow or cell plate, leading to the physical division of the cytoplasm and the creation of two distinct daughter cells.