How do you separate a mixture of two dyes using chromatography?

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1273976

2026-04-21 06:10

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To separate a mixture of two dyes using chromatography, you start by applying a small spot of the dye mixture onto a stationary phase, such as paper or a thin layer of silica gel. The stationary phase is then placed in a solvent, which acts as the mobile phase, and as the solvent travels up the stationary phase, it carries the dyes with it. Different dyes will move at different rates based on their solubility and affinity for the stationary phase, resulting in separation. Once the solvent front has reached a desired height, the paper or plate is removed, and the separated dyes can be visualized and analyzed.

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